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pckA [2019-08-09 07:48:10]
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pckA [2019-08-09 07:48:10]

phosphoenolpyruvate carboxykinase
Locus
BSU_30560
Isoelectric point
5.12
Molecular weight
58.13 kDa
Protein length
527 aa Sequence Blast
Gene length
1584 bp Sequence Blast
Function
synthesis of phosphoenolpyruvate
Product
phosphoenolpyruvate carboxykinase
Essential
no
E.C.
4.1.1.49
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Gluconeogenesis

    • Gene

    Coordinates
    3,129,530 3,131,113

    The protein

    Catalyzed reaction/ biological activity

  • ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)

    • Protein family

  • phosphoenolpyruvate carboxykinase (ATP) family (single member, according to UniProt)

    • Domains

  • Nucleotide binding Domain (233240)

    • Structure

  • 2PXZ (E.coli)

    • Localization

  • cytoplasm (according to Swiss-Prot), cytoplasm

    • Expression and Regulation

    Operons

    Genes
    pckA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpN: repression, PubMed, in CcpN regulon

    • Regulation

  • repressed by glucose (35-fold) CcpN PubMed
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    Biological materials

    Mutant

  • 1A1005 ( pckA::spec), [Pubmed| ], available at BGSC
  • 1A996 ( pckA::spec), [Pubmed| ], available at BGSC
  • GP1147 (pckA::neo), available in Jörg Stülke's lab
  • BKE30560 (pckA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAAACCTTCCTTTAT, downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG
  • BKK30560 (pckA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAAACCTTCCTTTAT, downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG

    • Expression vectors

  • pGP1753 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
  • pGP1762 (for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab)
  • pGP1763 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)

    • GFP fusion

  • GP1430 (spc, based on pGP1870), available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1129 (spc, based on pGP1331), available in Jörg Stülke's lab PubMed

    • Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

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